The oxidized DAB forms a brown precipitate, at the location of the HRP, which can be visualized using light microscopy. In DAB staining, DAB is oxidized by hydrogen peroxide in a reaction typically catalyzed by horseradish peroxidase (HRP). It is also used in in situ hybridization (ISH) and sometimes in dot blots and in western blotting. It is most often used in immunohistochemical (IHC) staining as a chromogen. Principles and application of DAB stainingĭAB (3,3′-Diaminobenzidine) is a derivative of benzene. Alternatively, you can assemble the reagents for DAB staining yourself, using biotin-conjugated secondary antibodies, HRP-polymer-conjugated secondary antibodies, and streptavidin-HRP conjugates. ![]() Linking HRP and primary antibodies for DAB stainingįor easy results with DAB staining, use a simple DAB substrate kit, or complete DAB staining kit, such as streptavidin-biotin HRP/DAB kits, and polymer method HRP/DAB kits.Principles and application of DAB staining.
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